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Biblioteca (s) : |
INIA Las Brujas. |
Fecha : |
12/11/2015 |
Actualizado : |
28/10/2019 |
Tipo de producción científica : |
Artículos en Revistas Indexadas Internacionales |
Autor : |
BENÍTEZ-GALEANO, M.J.; RUBIO, L.; BERTALMIO, A.; MAESO, D.; RIVAS, F.; COLINA, R. |
Afiliación : |
MARÍA JOSÉ BENÍTEZ-GALEANO, Universidad de la República (UdelaR)/ Centro Universitario Regional Noroeste, Salto (CENUR); LETICIA PAOLA RUBIO CATTANI, INIA (Instituto Nacional de Investigación Agropecuaria), Uruguay; ANA MARIA BERTALMIO CASARIEGO, INIA (Instituto Nacional de Investigación Agropecuaria), Uruguay; DIEGO CESAR MAESO TOZZI, INIA (Instituto Nacional de Investigación Agropecuaria), Uruguay; CARLOS FERNANDO RIVAS GRELA, INIA (Instituto Nacional de Investigación Agropecuaria), Uruguay; RODNEY COLINA, Universidad de la República (UdelaR)/ Centro Universitario Regional Noroeste, Salto (CENUR). |
Título : |
Phylogenetic studies of the three RNA silencing suppressor genes of south american ctv isolates reveal the circulation of a novel genetic lineage. |
Fecha de publicación : |
2015 |
Fuente / Imprenta : |
Viruses, 2015, v.7, no.7, p.4152-4168. OPEN ACCESS. |
ISSN : |
1999-4915 |
DOI : |
10.3390/v7072814 |
Idioma : |
Inglés |
Notas : |
Article history: Received: 26 May 2015 / Accepted: 17 July 2015 / Published: 22 July 2015. |
Contenido : |
ABSTRACT.
Citrus Tristeza Virus (CTV) is the most economically important virus of citrus worldwide. Genetic diversity and population structure of CTV isolates from all citrus growing areas from Uruguay were analyzed by RT-PCR and cloning of the three RNA silencing suppressor genes (p25, p20 and p23). Bayesian phylogenetic analysis revealed the circulation of three known genotypes (VT, T3, T36) in the country, and the presence of a new genetic lineage composed by isolates from around the world, mainly from South America. Nucleotide and amino acid identity values for this new genetic lineage were both higher than 97% for the three analyzed regions. Due to incongruent phylogenetic relationships, recombination analysis was performed using Genetic Algorithms for Recombination Detection (GARD) and SimPlot software. Recombination events between previously described CTV isolates were detected. High intra-sample variation was found, confirming the co-existence of different genotypes into the same plant. This is the first report describing: (1) the genetic diversity of Uruguayan CTV isolates circulating in the country and (2) the circulation of a novel CTV genetic lineage, highly present in the South American region. This information may provide assistance to develop an effective cross-protection program. |
Palabras claves : |
CITRUS TRISTEZA VIRUS; RT-PCR. |
Thesagro : |
CITRUS; CRUZAMIENTO INTERCROMOSOMICO; FILOGENIA; RECOMBINACION; URUGUAY. |
Asunto categoría : |
F30 Genética vegetal y fitomejoramiento |
URL : |
http://www.ainfo.inia.uy/digital/bitstream/item/5167/1/Rivas-F.-Maeso-D.-2015.-Viruses.pdf
http://www.mdpi.com/1999-4915/7/7/2814
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Marc : |
LEADER 02270nam a2200289 a 4500 001 1053851 005 2019-10-28 008 2015 bl uuuu u0uu1 u #d 022 $a1999-4915 024 7 $a10.3390/v7072814$2DOI 100 1 $aBENÍTEZ-GALEANO, M.J. 245 $aPhylogenetic studies of the three RNA silencing suppressor genes of south american ctv isolates reveal the circulation of a novel genetic lineage.$h[electronic resource] 260 $aViruses, 2015, v.7, no.7, p.4152-4168. OPEN ACCESS.$c4168 500 $aArticle history: Received: 26 May 2015 / Accepted: 17 July 2015 / Published: 22 July 2015. 520 $aABSTRACT. Citrus Tristeza Virus (CTV) is the most economically important virus of citrus worldwide. Genetic diversity and population structure of CTV isolates from all citrus growing areas from Uruguay were analyzed by RT-PCR and cloning of the three RNA silencing suppressor genes (p25, p20 and p23). Bayesian phylogenetic analysis revealed the circulation of three known genotypes (VT, T3, T36) in the country, and the presence of a new genetic lineage composed by isolates from around the world, mainly from South America. Nucleotide and amino acid identity values for this new genetic lineage were both higher than 97% for the three analyzed regions. Due to incongruent phylogenetic relationships, recombination analysis was performed using Genetic Algorithms for Recombination Detection (GARD) and SimPlot software. Recombination events between previously described CTV isolates were detected. High intra-sample variation was found, confirming the co-existence of different genotypes into the same plant. This is the first report describing: (1) the genetic diversity of Uruguayan CTV isolates circulating in the country and (2) the circulation of a novel CTV genetic lineage, highly present in the South American region. This information may provide assistance to develop an effective cross-protection program. 650 $aCITRUS 650 $aCRUZAMIENTO INTERCROMOSOMICO 650 $aFILOGENIA 650 $aRECOMBINACION 650 $aURUGUAY 653 $aCITRUS TRISTEZA VIRUS 653 $aRT-PCR 700 1 $aRUBIO, L. 700 1 $aBERTALMIO, A. 700 1 $aMAESO, D. 700 1 $aRIVAS, F. 700 1 $aCOLINA, R.
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Registro original : |
INIA Las Brujas (LB) |
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Biblioteca (s) : |
INIA Treinta y Tres. |
Fecha actual : |
10/12/2020 |
Actualizado : |
10/12/2020 |
Tipo de producción científica : |
Capítulo en Libro Técnico-Científico |
Autor : |
CEDRÉS, S.; ZORRILLA, G.; ROVIRA, A.; GUSSONI, A.; SANGUINETTI, M.; CARMONA, L. |
Afiliación : |
S. CEDRÉS, Técnico Regional Proyecto; G. ZORRILLA, Coordinador General Proyecto; Técnico Regional Proyecto; A. GUSSONI, Técnico Regional Proyecto; M. SANGUINETTI, Técnico Regional Proyecto; L. CARMONA, Consultor de Agronomía del FLAR. |
Título : |
Fortalecimiento de las estrategias de transferencia para reducir las brechas de rendimiento en el sector arrocero. |
Fecha de publicación : |
2020 |
Fuente / Imprenta : |
In: Terra, J. A.; Martínez, S.; Saravia, H.; Mesones, B.; Álvarez, O. (Eds.) Arroz 2020. Montevideo (UY): INIA, 2020. p. 71-73. |
Serie : |
(INIA Serie Técnica; 257) |
ISBN : |
e-ISBN: 978-9974-38-448-4 |
DOI : |
10.35676/INIA/ST.257 |
Idioma : |
Español |
Palabras claves : |
MANEJO EFICIENTE; MARGEN BRUTO; TRANSFERENCIA. |
Thesagro : |
ARROZ. |
Asunto categoría : |
F01 Cultivo |
URL : |
http://www.ainfo.inia.uy/digital/bitstream/item/14851/1/ST-257-p71-73.pdf
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Marc : |
LEADER 00814naa a2200241 a 4500 001 1061563 005 2020-12-10 008 2020 bl uuuu u00u1 u #d 024 7 $a10.35676/INIA/ST.257$2DOI 100 1 $aCEDRÉS, S. 245 $aFortalecimiento de las estrategias de transferencia para reducir las brechas de rendimiento en el sector arrocero.$h[electronic resource] 260 $c2020 490 $a(INIA Serie Técnica; 257) 650 $aARROZ 653 $aMANEJO EFICIENTE 653 $aMARGEN BRUTO 653 $aTRANSFERENCIA 700 1 $aZORRILLA, G. 700 1 $aROVIRA, A. 700 1 $aGUSSONI, A. 700 1 $aSANGUINETTI, M. 700 1 $aCARMONA, L. 773 $tIn: Terra, J. A.; Martínez, S.; Saravia, H.; Mesones, B.; Álvarez, O. (Eds.) Arroz 2020. Montevideo (UY): INIA, 2020. p. 71-73.
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